29. March 2019

Marloes Groot

Instant pathology with higher harmonic generation

Vrije Universiteit Amsterdam/ Amsterdam, The Netherlands

Intra-operative assessment of tissue, for example to determine whether excised tissue contains tumor cells or not, represents one of the major unmet-clinical needs at the moment. 
By an ‘instant’ pathology method the quality of surgery can be improved, the need for a second surgery minimized and correlation with pre-operative imaging methods may further enhance the interpretation of pre-operative images, and improve diagnosis. A technique that meets these requirements is third and second harmonic generation (THG/SHG) microscopy, whichis non-invasive, label-free and provides 3D images with a high, sub-cellular resolution, within seconds [1,2].

In my talk, I will present our results on human brain tumor samples, where normal brain was discriminated from infiltrative glioma with 96.6% sensitivity and 95.5% specificity, in nearly perfect (93%) agreement with pathologic scoring [3] and concurring results were reached in healthy breast tissue [4]. I will present artificial intelligence – based real time diagnosis of tissue, and I will present the first results of measurements taken with a transportable system in the lung surgery room. 

[1] S. Witte, A. Negrean, J.C. Lodder, C.P.J. de Kock, G.T. Silva, H.D. Mansvelder, and M.L. Groot, PNAS2011. 108(15): p. 5970-5975
[2] N.V. Kuzmin, P. Wesseling, P.C. Hamer, D.P. Noske, G.D. Galgano, H.D. Mansvelder, J.C. Baayen, and M.L. Groot, Biomed Opt Express2016. 7(5): p. 1889-904
[3] Z. Zhang, J.C. de Munck, N. Verburg, A.J. Rozemuller, W. Vreuls, P. Cakmak, L.M.G. van Huizen, S. Idema, E. Aronica, P.C. de Witt Hamer, P. Wesseling, and M.L. Groot, Advanced Science2019. 0(0): p. 1900163
[4] L.M.G. van Huizen, N.V. Kuzmin, E. Barbé, S. van der Velde, E.A. te Velde, and M.L. Groot, J. of Biophotonics,2019. 0(ja): p. e201800297