26. February 2019

Mischa Bonn

Quantitative Coherent Anti-Stokes Raman Scattering Microspectroscopy

Max Planck Institute for Polymer Research / Mainz, Germany

Owing to its ability to obtain molecularly specific contrast based on the vibrational spectra of molecules in the specific sample, Coherent Anti-Stokes Raman Scattering (CARS) microscopy has developed as a powerful label-free microscopy technique in the past decade. It has been challenging, however, to use CARS in a quantitative manner, especially for microscopy employing single-frequency CARS. 

One of the key advantages of multiplex CARS in combination with appropriate data analysis tools is the possibility to monitor and quantify small changes in the local chemical composition of a sample with submicron resolution. The non-resonant background is used to heterodyne the CARS signal, and a simple phase retrieval method provides the corresponding, quantitative, local Raman response. We report the use of quantitative multiplex CARS microscopy to several challenges in material- and bio-sciences.